Monoploid genome proportions (1Cx-values) and you may ploidy membership were not inferred for A great

Monoploid genome proportions (1Cx-values) and you may ploidy membership were not inferred for A great

fibrillosum, A great.soleirolioides and you can A good.polyphyllum, as a result of the shifts noticed in the fresh new 2C DNA belongings in such variety, as a consequence of its storage for a couple of years during the silica (see over). Ploidy account and you may 1Cx-thinking were inferred for everyone other subclades analysed (Desk 4 and you can Shape 3).

Like, compared to the small 1Cx-beliefs on A great

A summarized phylogenetic tree of your own Asplenium monanthes state-of-the-art and you will associated lineages [due to the fact presented for the 30] together with the inferred ploidy account (based on composed chromosome study), mean holoploid genome size (1C-value; grey pubs), and inferred monoploid genome proportions (1C x -value; black taverns) while the mean spore size (?m) of each and every operational unit. Posterior branch service >0.8 is found. Getting diploids only black colored bars occur once the 1C-worthy of is equal to the fresh new 1Cx-value. Standard mistake taverns come for 1C-value and you may 1Cx-worthy of function, apart from lineages depicted by the one specimen just. Forest is actually grounded based on Dyer mais aussi al. . Specifics of the container plots of land to possess spore dimensions are supplied inside the the newest legend to find step one.

When you look at the Good.resiliens clade, different ploidy accounts was indeed inferred into the one or two A.aff.heterochroum specimens. One specimen of An excellent.aff.heterochroum (RD75; 2C = pg) got a similar 2C-really worth in order to A great.resiliens (indicate 2C = pg), a variety considered to be triploid, recommending you to definitely RD75 may be a beneficial triploid. Alternatively, additional Good.aff.heterochroum sample analysed (RD9a) had a somewhat high 2C-value ( pg) and you can an effective 1Cx-worthy of approximately fourfold one to inferred to have RD75, and therefore resulted in inference this particular sample was a good tetraploid.

The fresh new tip that every An effective.monanthes specimens was triploid is dependant on the fresh new observation one, with the exception of one statement regarding a beneficial tetraploid count, most of the counts for it variety were 2n = 3x = 108. Lower than it expectation, the brand new indicate 1Cx-thinking having MO1 sub-clades I, II and III are pg, 9.33 g, and 8.95 pg, respectively (Desk 4). Good triploid peak is also presumed to have MO2 (I), in line with the 2C-opinions, causing a hateful 1Cx-worth of 9.02 pg. Brand new An effective.monanthes MO2 (II) sub-clade, and therefore comprised just one sample (RD96), got a considerably lower 2C-property value pg, therefore infer that sample is likely to be a beneficial diploid cytotype. This would lead to a great 1Cx property value nine.31 pg, which is within the wider contract to your monoploid genome products calculated toward left A beneficial.monanthes sandwich-clades (Dining table cuatro).

Into the A.hallbergii, the newest resemblance of their indicate 2C-worthy of ( pg) and mean spore proportions ( ?m) compared to that of triploid A good.monanthes lineages (MO1 and MO2.I) means which types is even good triploid. When it comes to a couple of the new kinds, spec.nov.1 and you can specification.nov.dos, they differ into the 2C-values ( pg and you can pg correspondingly) but i have likewise small spore systems. They are already inferred are diploid even though further research is actually must prove or reject this.

Nevertheless, it’s indexed that imply spore size of RD96 ( ?m) disputes using this type of shopping for, suggesting that sample was a great polyploid

Total, the outcome demonstrate that monoploid genome dimensions may differ dos.4-bend (4.66- pg), with little to no variation inside 1Cx-thinking within this clades, however, considerable variation between many of them (Desk cuatro and you may Figure step three). resiliens clade (cuatro.66-4.79 pg), the latest suggest 1Cx-worth for diploid An effective.formosum (six.81 pg) was markedly large. A bigger monoploid genome dimensions has also been indexed on the A great.monanthes clade weighed against the brand new A.resiliens clade. In fact, the largest monoploid genome types was basically came across inside A great.monanthes clade (8.70- pg), with spec.nov.2 having the large 1Cx-value ( pg) of all of the, that is more than double the mean monoploid genome sized the A beneficial.resiliens clade.


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